Review



stringency wash buffer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Thermo Fisher stringency wash buffer
    Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/SSC+buffer/bio_rxiv__64898__2026__04__07__716965-142-5-10
    Average 97 stars, based on 1 article reviews
    stringency wash buffer - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: ZNF692 organizes a hub for ribosome maturation enhancing translation in rapidly proliferating cells
    Article Snippet: Membranes were prehybridized with ULTRAhyb buffer (Northern Max Kit, Invitrogen) for 30 min at 37°C and hybridized overnight with each probe (10 μM, 1:1000) in ULTRAhyb buffer at 37°C. .. After washing with Stringency wash buffer (2X SSC and 0.5% SDS) twice and Wash buffer (0.5% SDS and 1X PBS) twice, membranes were blocked with Blocking buffer (0.5% SDS, 1% BSA and 1X PBS) for 30 min, followed by incubation with HRP-Conjugated streptavidin (Thermo Scientific, 1:4000) for 1 hr at 37°C. ..

    Incubation:

    Article Title: ZNF692 organizes a hub for ribosome maturation enhancing translation in rapidly proliferating cells
    Article Snippet: Membranes were prehybridized with ULTRAhyb buffer (Northern Max Kit, Invitrogen) for 30 min at 37°C and hybridized overnight with each probe (10 μM, 1:1000) in ULTRAhyb buffer at 37°C. .. After washing with Stringency wash buffer (2X SSC and 0.5% SDS) twice and Wash buffer (0.5% SDS and 1X PBS) twice, membranes were blocked with Blocking buffer (0.5% SDS, 1% BSA and 1X PBS) for 30 min, followed by incubation with HRP-Conjugated streptavidin (Thermo Scientific, 1:4000) for 1 hr at 37°C. ..

    Labeling:

    Article Title: Lipid kinase PIP5K1A regulates let-7 microRNA biogenesis through interacting with nuclear export protein XPO5.
    Article Snippet: Before hybridization, the membranes wer e pr e-hybridized for at least 1 h at 40 ◦C in pre-hybridization buffer (#AM8677, Thermo Scientific). .. Next, hybridiza tion buf fer containing 50 pmol / ml biotin labeled single-stranded DNA oligonucleotide (See below) was added and the membranes were hybridized for overnight at 40 ◦C with gentle shaking and subsequently rinsed with low stringency wash buffer (#AM8673, Thermo Scientific) 3 times and High stringency wash buffer 2 times (#AM8674, Thermo Scientific) at room temperature. .. The biotin-labeled probes were detected using a Chemiluminescent Nucleic Acid Detection Module Kit (#89880, Thermo Scientific) following the manufacturer’s instructions.

    Gentle:

    Article Title: Lipid kinase PIP5K1A regulates let-7 microRNA biogenesis through interacting with nuclear export protein XPO5.
    Article Snippet: Before hybridization, the membranes wer e pr e-hybridized for at least 1 h at 40 ◦C in pre-hybridization buffer (#AM8677, Thermo Scientific). .. Next, hybridiza tion buf fer containing 50 pmol / ml biotin labeled single-stranded DNA oligonucleotide (See below) was added and the membranes were hybridized for overnight at 40 ◦C with gentle shaking and subsequently rinsed with low stringency wash buffer (#AM8673, Thermo Scientific) 3 times and High stringency wash buffer 2 times (#AM8674, Thermo Scientific) at room temperature. .. The biotin-labeled probes were detected using a Chemiluminescent Nucleic Acid Detection Module Kit (#89880, Thermo Scientific) following the manufacturer’s instructions.



    Similar Products

    94
    Zymo Research high stringency wash buffer
    High Stringency Wash Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/M-Wash+Buffer/pm40185099-433-38-74
    Average 94 stars, based on 1 article reviews
    high stringency wash buffer - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    97
    Thermo Fisher stringency wash buffer
    Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/SSC+buffer/bio_rxiv__64898__2026__04__07__716965-142-5-10
    Average 97 stars, based on 1 article reviews
    stringency wash buffer - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher stringency wash
    Stringency Wash, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/SSC+buffer/pmc12483275-294-58-70
    Average 99 stars, based on 1 article reviews
    stringency wash - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    94
    Zymo Research low stringency wash buffer
    Low Stringency Wash Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/M-Wash+Buffer/pm40185099-433-10-74
    Average 94 stars, based on 1 article reviews
    low stringency wash buffer - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    90
    Thermo Fisher hybridization stringency wash buffer (2×) north2south
    Reagents and tools table
    Hybridization Stringency Wash Buffer (2×) North2south, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/north2south+chemiluminescent+hybridization+and+detection+kit/pmc12048567-58-0-8
    Average 90 stars, based on 1 article reviews
    hybridization stringency wash buffer (2×) north2south - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher north2south (r) hybridization stringency wash buffer (2x
    Reagents and tools table
    North2south (R) Hybridization Stringency Wash Buffer (2x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stringency+wash+buffer/pm39579766-594-282-289
    Average 90 stars, based on 1 article reviews
    north2south (r) hybridization stringency wash buffer (2x - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: A double-agent microRNA regulates viral cross-kingdom infection in animals and plants

    doi: 10.1038/s44318-025-00405-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: North2South TM Hybridization Stringency Wash Buffer (2×) , Thermo Scientific , Cat# 37555.

    Techniques: Virus, Recombinant, FLAG-tag, Sequencing, Labeling, Protease Inhibitor, Reverse Transcription, Membrane, Transfection, Hybridization, Blocking Assay, Caspase-3 Activity Assay, Activity Assay, TUNEL Assay, Apoptosis Assay, Fluorescence, Luciferase, SYBR Green Assay, Expressing, Cloning, Extraction, Transformation Assay, Immunoprecipitation, Software